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Immunohistochemistry (IHC) Fresh human specimens were fixed with 4% paraformaldehyde solution for 24 h, embedded in paraffin, and cut into sections (5 m), which were then immersed in xylene and ethanol for deparaffinization and rehydration, respectively

These data also support the conclusion that Fab 3F52 directly blocked binding of BMS21, implying that Fab 3F52 occupies partially the orthosteric binding site of the GLP-1R TM domain, which is interesting considering the spatial relationship of the ECD, the antibody and the TM domain

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It is stored mainly in skeletal muscle, brain and heart, where it is needed for energy production from fatty acids

doi: 10.1016/S2213-8587(17)30013-X 5