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The most important thing to remember here is to be gentle

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Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

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and one-pot assembly, which involves incorporating ligand-conjugated lipids during liposome formation

Antioxidant functions of vitamin D and CYP11A1-derived vitamin D, tachysterol, and lumisterol metabolites: mechanisms, clinical implications, and future directions