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Brain sample collection and sectioning Mice were euthanized with isoflurane (5%, Panion & BF Biotech), and subsequently perfused transcardially with PBS and paraformaldehyde (4% w/v, Electron Microscopy Sciences) for brain collection

Other treatment options have traditionally included surgical removal (excision), liquid nitrogen (freezing it off), curettage (scraping it off, often with electrical controlled burning), and lasering it off

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Brain Behavior and Immunity, 106088106088

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Effects of maternal smoking during pregnancy on offspring externalizing problems: contextual effects in a sample of female twins