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We then intercrossed the F1 offspring to generate the following F2 offspring: Nestin-Cre ER :: Igf1r fl/fl (homozygous IGF1R inducible knockout mice, hereafter referred to as MSC- Igf1r / ), Nestin-Cre ER :: Igf1r fl/+ (heterozygous IGF1R inducible knockout mice), and mice without either the Nestin-Cre ER or a floxed Igf1r allele (hereafter referred to as wild type) (Figure 2)

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