Then, cells were incubated with a 1:200 dilutions of primary anti--H2AX antibody (ABclonal Technology, Wuhan, China) overnight at 4 C, followed by three washes with PBS and incubation with FITC-labeled goat anti-rabbit IgG antibody for 1 h and mounted with DAPI [32]
[DOI] [PMC free article] [PubMed] [Google Scholar] 65.Rees H.A., Minella A.C., Burnett C.A., Komor A.C., Gaudelli N.M
Front Oncol 3:326 Paradells-Navarro S, Benlloch-Navarro MS, Almansa Frias MI, Garcia-Esparza MA, Broccoli V, Miranda M, Soria JM (2017) Neuroprotection of brain cells by lipoic acid treatment after cellular stress
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