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Most injection site reactions are mild and self-limiting, requiring only observation and basic symptomatic management

Subsequently, the tissue fragments were inoculated into T75 culture flasks and, once the tissue adhered, the medium was replaced with complete medium (MEM, 20% FBS, 1% PS, 2 g/mL epidermal growth factor EGF, 25 ng/mL fibroblast growth factor FGF) to facilitate cell migration

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10.1016/0014-5793(93)80212-d 81 NakamuraT.NawaK.IchiharaA.KaiseN.NishinoT

doi: 10.1093/jn/127.12.2274 251 SchuschkeD

This response was attributed to microevolutions through mutations in icc which result in epigenetic and morphological adaptations that contribute to persistence and disease severity (Hardison et al., 2018a)