On the surface, it seems like a minor chemical substitution
For a 10 mg vial of 5-Amino-1MQ, reconstitute with 3.0 mL of bacteriostatic water to achieve a concentration of approximately 3.33 mg/mL
Let the water run slowly down the inner glass wall never spray directly onto the lyophilized powder
Bacteriostatic water contains 0.9% benzyl alcohol, a preservative that prevents bacterial growth, making it suitable for multi-use vials

related full-length recombinant thymosin beta-4 (a different formulation than the marketed TB-500 fragment) has also been examined in small human studies for cardiac and dermatologic indications Examined in preclinical models, including diabetic rat Achilles tendon and rat ligament-injury studies, for actin-binding, cell-migration, and angiogenic activity that researchers have proposed as a mechanism for tendon and ligament repair, though this literature remains comparatively sparse for musculoskeletal applications specifically Known risks and side effects: Because TB-500 is manufactured and sold without FDA oversight as a "research chemical," independent testing and industry reporting describe wide variability in purity (with some products measured well below labeled potency) and contamination with heavy metals or bacterial byproducts, unlike pharmaceutical-grade recombinant thymosin beta-4 used in registered clinical trials Thymosin beta-4's core mechanisms, angiogenesis and cell migration, are also pathways exploited by tumors

The trials lasted 16 days