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GSH Quantification Glutathione and GSSG were measured in lysates from cells in 6-well plates as previously described ( ROS Quantification by Spin-Trapping and EPR Spectroscopy Cells (5 10 6 /0.1 ml) were stimulated for 2 h with LPS, then incubated with 50 mM 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO), synthetized as described previously ( Cell Viability Assay Cells were seeded in 96-well plates at 25,000/well in complete medium

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Furthermore, we found using neural cell cultures that synthetic DOPA pheomelanin induced neuronal cell death, while synthetic DOPA eumelanin showed no significant effect on cell viability, and believe this may reflect properties of the pheomelanic and eumelanic components of natural NM (Cai et al

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