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GC/MS analysis Extract (1 ml) was injected immediately after rotovapping into a Thermo Trace GC/Polaris Q GC/MS containing a 30-m Restek Rxi-5ms fused silica column

Incorrect procedures compromise experimental results and introduce potential hazards

Severe side effects are rare but include anaphylaxis (especially in those allergic to cobalt or B)

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Or split the weekly dose into two smaller injections (for example, two 0.125mg injections instead of one 0.25mg injection)