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This solution was sterile filtered through a 0.2 filter and slowly added to a stirred diethylether (50 ml)

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Interestingly, the purified COP1-c-Jun complex also yielded detectable c-Jun ubiquitination, although to a lesser extent than reactions containing the full CRL4 DET1-COP1 complex with Ube2d3 (Fig

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PMID: 22172216