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Autophagic vesicles on mature human reticulocytes explain phosphatidylserine-positive red cells in sickle cell disease

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For pdbqt preparation, the prepare_ligand4.py script from AutoDockTools 1.5.7 (Trott & Olson, 2010) was employed, retaining all atoms without cleanup (-U) while repairing hydrogens (-A) to ensure compatibility with docking calculations

The answer above covers storage requirements, but misses the mechanism that makes temperature control non-negotiable

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