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The synergistic effect of tirzepatide and leptin on weight loss in mice To evaluate the combination effect of tirzepatide and leptin, the DIO mice received daily subcutaneous injections of tirzepatide (TZP, 3 nmol/kg), a mouse IgG1-Fc and human leptin fusion protein (FcLep see methods, 40 nmol/kg), or a combination of tirzepatide and leptin (TZP+FcLep) for three weeks, following 5-day diet switch acclimation and 3 daily vehicle (40 nM Tris, pH 8) injection acclimation (Fig

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