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5,10-Methylene tetrahydrofolate (THF) is required for the synthesis of nucleic acids and is reduced to 5-methyl THF by methylene THF reductase, a flavin-dependent enzyme that uses NADPH as a cofactor

In contrast, the thiyl radical propagates radical chain reactions 34,35 , and even the long-lived -tocopherol radical does so 36

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Protein assays based on Coomassie blue give the most reliable data