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For each plate, the concentration of product was normalized by assigning a score of 0 (0%) to the negative control (average of maximal concentration of product measured in the presence of enzyme without any inhibitor) and a score of 1 (100%) was assigned to the positive control (average minimal concentration of product measured in the presence of enzyme with the reference inhibitor, i.e., EA)

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41,48,49 It has been reported that up to 75% of their metabolism is unproductive due to proton leak (premature leakage of protons through the inner mitochondrial membrane not mediated by ATP synthase) which is staggering, given that insulin secretion occurs in an ATP-dependent manner

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