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Recombinant baculovirus was produced by transfecting Sf9 cells with the bacmid DNA using Cellfectin (Cellfectin Reagent Invitrogen)

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Although functional data supporting a trans- inhibition model were obtained when constitutive activation of JAK2 was shown with a JAK2 construct where the pseudokinase and KDs were swapped and this construct showed significant cytokine-independent activation only in the presence of wild-type JAK2 (51)

For women with insulin resistance, which is common in polycystic ovary syndrome (PCOS), prediabetes, and obesity, this effect is particularly meaningful

These symptoms may indicate serious complications requiring immediate medical intervention