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Cell culture and transfection WT HepG2 or stably transfected cells (pSuperior.puro-GSTM2) were cultured in DMEM (HyClone, Logan, UT, USA) containing 10% foetal bovine serum (Clark Bioscience, Richmond, VA, USA), 100 U/mL penicillin, and 100 g/mL streptomycin in dishes at 37 C and transfected with Lipo6000 Transfection Reagent (#C0528, Beyotime)

at a concentration of 4 mM with an injection volume of 100 L/20 g body weight 1 h prior to the administration of PHZ

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The TLR3 agonist poly (I:C) prevents DSS-induced colitis (Zhong and Kyriakis, 2004)

Q: How does GHK-Cu compare to other peptides for skin and hair concerns

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