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In contrast to the cortical cataract, nuclear cataracts tend to become harder and less hydrated than normal, age-matched lens nuclei

After fixation, coverslips were incubated in a blocking solution containing 5% bovine serum albumin (BSA), 0.01% Triton X-100, and 3% goat serum for 2 h and 30 min, adapted from Orellana and colleagues 41

The results provide additional evidence that fork degradation in the absence of PDS5B is linked to RAD51-mediated fork reversal, in that cells expressing the RAD51 K133R or G151D mutant were susceptible, while cells that expressed the RAD51 II3A and T131P mutants were not (Fig

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Storage and handling post-mixing are vital

Moore J