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GHK-Cu and vitamin C should not be applied in the same session because ascorbic acid can reduce copper ions and diminish GHK-Cus activity

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Samples were incubated at room temperature for 1 h, vortexing intermittently, and then quenched by the addition of hydroxylamine (5 L of 5% w/v in HPLC water per sample) and incubated for 15 min at room temperature

10.1111/j.2042-7158.1990.tb07021.x 192 RavikumarH.MuralidharaG