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Extracellular vesicle heterogeneity: subpopulations, isolation techniques, and diverse functions in cancer progression

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The complex was then concentrated using an Amicon Ultra Centrifugal Filter (MWCO, 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20mM HEPES pH 7.4, 100mM NaCl, 2mM MgCl 2 , 1M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS to separate complex from contaminants

Figure 3 shows the distribution of the total number of subjects in the studies included in this review

For cross-site replication, methods sections include exact reagent names, concentrations, and assay lot numbers so others can reproduce exposure-response relationships under matched conditions

The results of this study showed that C