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Procedures Blood collection and centrifugation Animals were anesthetized with isoflurane and whole blood collected via cardiac puncture

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I have completed 3 of the 4 first month injections and have lost 6 pounds

Real-time PCR Total RNA was isolated from the cells using the Roche High Pure RNA isolation kit (Roche Life Science, 11828665001) and reverse transcription was performed using the iScript cDNA synthesis kit (Bio-Rad, 1708891) according to the manufacturers instructions