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Graphical Abstract Keywords Transaminases, Vitamin B 6 , Celiac disease, Renal insufficiency, Hepatolenticular Degeneration, Crohn disease, Malnutrition, Clofibrate, Bone diseases, Lead poisoning, Anemia, Hemolytic Introduction Liver disease is often detected using automated assays of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyl transpeptidase (GGT) and 5-nucleotidase (5-NT)