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Both RAW 264.7 cells and peritoneal macrophages were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum, 5% CO, and 95% air at 37C

J.LingG.WakabayashiN.et al

This approach is particularly beneficial in trials involving multiple treatment arms or stratified subgroups, where conventional fixed designs often prove too rigid or inefficient