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doi: 10.1016/S0010-7824(02)00277-9 22 HuangY-YWuC-HLiuC-HYangS-FWangP-HLinL-Yet al

Bacteriostatic water offers several advantages over alternatives: pH range of 5.0-7.0 prevents acid- or base-catalysed hydrolysis Benzyl alcohol at 0.9% concentration inhibits bacterial growth without denaturing peptides Multi-dose vials remain stable for 28 days under refrigeration (2-8C) No ionic species that could interfere with downstream analytical techniques (HPLC, mass spectrometry) Some UK suppliers provide pre-mixed solutions, but these introduce variables that complicate dose precision

The columns were pre-equilibrated with a buffer containing 20 mM HEPES (pH 7.0) and 150 mM NaCl

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Protocols in experimentation have been around 2 mg to 10 mg per day