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As another model of cell hypertrophy, hiPSC-CMs were seeded on Matrigel-coated 6-well Bioflex plates at 750,0001,000,000 cells/well and exposed to cyclic mechanical stretch by applying vacuum suction with an FX-5000 Tension System (Flexcell International Corporation, Hillsborough, NC, USA)

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Importantly, however, unlike nuclear MITF, we hardly observed significant differences in nuclear YAP when comparing the three ECM types, making it unlikely that MCs utilize YAP for ECM-specific melanin production

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