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doi: 10.3389/fimmu.2017.01368

[DOI] [PMC free article] [PubMed] [Google Scholar] 97.Zehou O., Fabre E., Zelek L., Sbidian E., Ortonne N., Banu E., Wolkenstein P., Valeyrie-Allanore L

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Maisch, r119.aq) and heated to 50 C using column heater sleeves (PhoenixST)

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Tissues subjected to repeated freeze-thaw cycles were homogenized at 20 L/mg tissue of cold water in a 2 ml Eppendorf tube using a metal bead beater operating at 30 Hz for 5 min in a TissueLyser III (Qiagen)