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Accordingly, alanine mutagenesis of TM7 residues influenced both GLP-1 and oxyntomodulin affinity, but there was a larger effect on oxyntomodulin, consistent with its stronger interactions, however, interestingly these residues were more important for GLP-1-mediated cAMP production (Supplementary Figs 9, 10)

As such, synthetic GLP-1/GCG co-agonists resistant to dipeptidyl peptidase-4 (DPP-4) proteolysis became an attractive target for anti-obesity treatments

Days 6 to 7 post-injection represent the waning period

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