Kinetic characterization of LaccID To further characterize the catalytic activity of LaccID, we purified recombinant enzyme from yeast S
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(6) in the passive skin allergy test of the high-stability reduced glutathione for injection, after the guinea pig is excited, the diameter of blue spots of a tested group and the absorbance of leachate have no difference compared with a negative group, and have obvious difference compared with a positive group, which indicates that the high-stability reduced glutathione for injection (with the batch number of YJ110401) does not generate passive skin allergy reaction on the guinea pig
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21 Among these precursors, NAM could be recycled from NAD + consumption reactions, including both NAD + -dependent deacylation and ADP-ribosylation, into NMN by NAMPT, which catalyzes the rate-limiting reaction in the salvage pathway