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Neuromasts were masked using a line tool and mean fluorescence was measured for an entire neuromast using ImageJ version 1.48 (National Institutes of Health, Bethesda, MD), mean background fluorescence was then subtracted out to yield net mean fluorescence for each neuromast
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In normal muscle stem cells, basal levels of autophagy contribute to the maintenance of stemness and pluripotency
Because the maturation cycle for eggs and sperm is roughly 90 days, it usually takes consistent support over several months to see the full impact on reproductive cell quality
