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In vitro binding assays using recombinant proteins Saturating amounts of clarified bacterial lysates containing GST and GST-S fusions were added to Glutathione Sepharose beads that were previously washed with lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 1 mM EDTA, 5 mM 2-mercaptoethanol, 1% Triton X-100) and incubated at 4 C for 1 h on a tube roller

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(28) The crude incidence rates of LLA were 6.1 per 10,000 person-years for GLP-1 receptor agonists, 1.5 for SGLT-2 inhibitors, 4.6 for DPP-4 inhibitors, and 1.9 for older agents

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