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The proteome concentration of the tissue lysates was determined using the DC protein assay (Bio-Rad), normalized to 2 mg/mL, and then 100 L of each sample was transferred to a LoBind Eppendorf tube containing 48 mg of urea

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doi: 10.1371/journal.pone.0075559 132 XuC.FanL.LinY.ShenW.QiY.ZhangY.et al

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The role of the kynurenine pathway in suicidality in adolescent major depressive disorder