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Commonly handled under cold-chain laboratory conditions, with frozen storage and light protection used to preserve sample integrity throughout the research period

Melatonin represses oxidative stress-induced activation of the MAP kinase and mTOR signaling pathways in H4IIE hepatoma cells through inhibition of Ras

The phosphorylation status of IB- and total cytosolic IB- levels in HepG2 cells were determined by western blot analysis using cytosolic protein fractions

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