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Microglia in the brain of the elderly are often characterized by increased activation and decreased regulatory status

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They are crucial components of brain cell membranes and play a vital role in neuronal communication

After treatment with the blocking solution (0.3% Triton-X and 3% BSA in 0.01 M PBS) to block non-specific staining and increase permeability to antibodies, the brain sections were incubated with anti-c-Fos rabbit polyclonal antibodies (abcam) in the blocking solution at 4 C overnight

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