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For unsecreted and DTC-associated basement membrane COL IV measurements, with the same images used for DTC-AC quantification, 3D models were generated based on the red EMB-9::mCherry marker using identical settings that allowed for consistent and specific labelling of the DTC intracellular punctuate signals that are referred to as blobs in the manuscript

Typically, cells were seeded in 100 L RPMI phenol red free media per well in duplicates on a black 96-well plate with clear bottom (Thermo Scientific)

Often called the "master antioxidant," glutathione is a molecule found in every cell in your body