Bacteria or cells Amount of cells (104): Extract solution (mL): 500~1000:1 (Add 1mL of Extract solution to 5 million cells), ultrasonic with ice bath to break cells(300W,3s, interval 7s,total time 3 minutes)
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Document both diagnoses clearly
Rubric Conclusion Time to celebrate your accomplishments
As expected, VEGF as positive control had proangiogenic effect, as opposed to negative control Suramin which practically completely inhibited branching ( Fig
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