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183,184 Human hepatocyte-differentiated organoids and liver-derived intrahepatic cholangiocyte organoids can recapitulate some pathologies of fatty liver disease, such as lipid accumulation and mitochondrial damage

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Interestingly, berberine activates AMPK by partially inhibiting mitochondrial complex I, which raises the AMP/ATP ratio inside cells a signal that tells the body, "energy is scarce, start burning fat." This is very similar to what happens during caloric restriction or exercise at the cellular level

YW and QT critically revised the manuscript