Sensitive and accurate: linear detection range 0.4 - 100 M in 96-well plate Simple and convenient: mix the DTNB reagent with sample, remove protein precipitates for proteinaceous samples, add a second reagent and read the optical density Low interference: amino acids and common buffers do not interfere Direct assays: reduced glutathione in whole blood, plasma, serum, urine, tissue and cell extracts Drug discovery/pharmacology: effects of drugs on glutathione metabolism Note: The below protocol is a sample protocol
Restoring GSH level is a good tool to deal with the above states but direct oral supplementation with GSH is not very efficient because of its poor absorption and because of its hydrolytic degradation in the digestive system
Taking these supplements at different times may enhance absorption and effectiveness
doi: 10.1016/j.bbagen.2012.11.020 16
Zhao, X., Liu, L., An, T., Xian, M., Luckanagul, J
Ignoring other antioxidants: Glutathione works best when combined with supporting nutrients like vitamin C and selenium
