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After sonicating (1400 rpm, 20 C, 5 min), the extracts were cleared by centrifugation (17,000 g , 20 C, 10 min), and the lipids in the supernatant were analyzed by LC-MS

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Here, LC, functioning as an essential cofactor and metabolic regulator, directly counteracts core pathological processes by enhancing mitochondrial -oxidation and attenuating oxidative stress