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Briefly, 5 L serum (after tenfold dilution with saline) was added to the total reaction system containing GSSG, NADPH and DTNB (5,5-dithiobis-(2-nitrobenzoic acid)

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First, we used a manual software (XsOs) to process (baseline correct and phase) our GSH spectra (manual processing yielded higher quality spectra than automatic processing software)