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Kit Components Additional Materials Required Microplate reader capable of measuring absorbance at 405 nm Precision pipettes to deliver 2 L to 1 mL volumes Multi-channel pipettes to deliver 20 L to 200 L volumes Tubes to prepare sample dilutions Ultrapure water 50 mL conical tubes Protocatechuic acid (HClO 4 ) (PCA) Potassium hydroxide (KOH) Assay Procedure Summary Add 50 L of the diluted GSSG Standards or unknown samples to the 96-well microtiter plate