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doi: 10.1016/j.cmet.2020.11.017 81 ZhangZGuoXLiuJGuYJiXZhuSet al
Total Thiols For determination of the total thiols, 200 L of tissue homogenate was mixed with 15 L of 0.25 M TCEP (dissolved in 0.2 M phosphate buffer, pH 8.2 and adjusted to pH 78 with 2.5 M NaOH) and the mixture was incubated at a room temperature for 15 min to reduce disulfide bonds
H+, Hydrogen ions
The peptide accelerates biological healing, but complete recovery requires addressing biomechanical factors, building strength, and optimizing the environment for tissue repair
In fact, reactive oxygen species (ROS) and peroxides (H2O2) upregulate the transsulfuration pathway (homocysteine towards cysteine and eventually the GSH-Px system) and downregulate the transmethylation (homocysteine towards methionine) (Figure 3)
Starting with careful dose titration minimises side effects while establishing sustainable habits
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