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Resistance T-47D (resistance human breast ductal carcinoma) was developed by gradually exposed to doxorubicin (DOX) to develop a resistant subline

The main enzyme involved, dipeptidyl peptidase-4 (DPP-4) also known as CD26, cleaves off the two N-terminal residues resulting in GLP-1 (9-36 amide) which is inactive or acts as an inhibitor at the GLP-1 receptor (23)

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We have implemented controls in our methods to counter adsorption that we see consistently for all peptides but it can be particularly problematic for peptides with lipid additions