10 (8), e01726
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At this stage, 2,271 studies were excluded because they did not meet the predefined eligibility criteria, leaving 231 articles for full-text evaluation
We first generated all single-step mutations from each training-set sequence in the three groups of interest, removing any duplicates within the generated set, and any overlaps with the training set
If it has been warm (above 25 degrees Celsius) for more than 24 hours, assess the situation carefully