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For the mice more than 100 days old, the remaining dermal tissue was digested additionally in the collagenase (Sigma) for 1 hr and then washed with PBS before imaging to allow detection of fluorescence signal

6,7 Specifically, Hagen et al

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Many people spend months applying different creams and products, only to watch the rash worsen

*Correspondence: Hongfeng Yang, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

Step 3: Pour into the mold Pour the soap into your mold and set aside to cool