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deSUMOylation of human RanGAP1-SUMO1 To demonstrate the ability of SnCE1 to cleave SUMO directly from protein substrates and not only poly-SUMO conjugates, 48 nM Hs RanGAP1-SUMO1 was incubated with 144 nM wild type SnCE1 or the inactive mutant SnCE1 C256A at room temperature as described before 110

The same sample volume and measurement settings were used as described above