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doi:10.1152/ajpendo.00249.2020 21

A protein rich meal can significantly raise total homocysteine levels, so clinicians generally recommend that the person taking the test should be overnight fasted at the time of blood sampling

In other words, it must be determined that the benefit/risk is highly favorable

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The flow rate was maintained at 0.15 ml min 1 with the following linear gradient: 023 min from 95% buffer B to 5% buffer B, 2325 min hold at 5% buffer B, 2525.5 min transition to 95% buffer B at 0.20 ml min 1 , 25.532.5 min hold at 95% buffer B and 32.533 min at 95% buffer B with a 0.15 ml min 1 flow rate