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To evaluate the information content generated by the sequence design process, we calculated the entropy across each set of designed sequences, and the relative entropy (KullbackLeibler divergence, KL) between the distribution of amino acids at each sequence position estimated for the model-designed samples, and the training data

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For the induction of gene expression, 0.050.5 mM isopropyl -D-1-thiogalactopyranoside (IPTG) was added as indicated in different experiments

Roux KJ, Kim DI, Burke B, May DG