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In contrast, good fits with concentration-independent rate constants were obtained for the monothiol mechanism (iii) from Fig

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The cells were resuspended in complete medium (RPMI-1640 for BxPC-3, DMEM for HPDE6-C7), supplemented with 10% fetal bovine serum and 1% penicillinstreptomycin, and diluted to a density of 3 10 4 cells/mL