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NO can also reversibly nitrosylate cysteine residues and inhibit mitochondrial cytochrome c, leading to increased production of superoxide that locally reacts with NO to form peroxynitrite (the extremely reactive nucleophile ONOO-) (Moncada and Bolaos, 2006
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A broken seal or signs of leakage Check that the cap and stopper are intact, with no cracks, leaks, or liquid pooled around the top, since a broken seal can mean contamination
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