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Twenty-four hours later, media was exchanged with 3 mL of DMEM +dFBS (or RPMI +pyruvate +dFBS / McCoys 5A +dFBS ) and 300 ng/mL of Dox (no Dox was added to 786-O lenti cell lines which express LUC or 87 Cr GPDH 705 constitutively), and cells were then returned to the incubator

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It reflects the biochemistry of copper peptides in solution

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The D-Retro-Inverso modification ensures the peptide maintains its binding affinity while resisting proteolytic degradation, allowing extended therapeutic activity for senescent cell targeting in complex tissue environments

The decision comes down to where you are in your dosing protocol