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This increase then elevates the levels of intracellular calcium by mobilizing the calcium stores found in the endoplasmic reticulum

Finally, the protein was purified by size-exclusion chromatography (GE HiLoad 16/60 Superdex 75 pg) in a final buffer containing 20 mM HEPES pH 7.5, 100 mM NaCl, 2 mM MgCl 2 , 10 M GDP, 1 mM TCEP, and 5 % glycerol

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